| Argyll Wildlife Park | Inveraray | Argyll and Bute | Wildlife Park |
| Aros Experience, The | Portree, Isle of Skye | Highland | Visitor Centre |
| Black Isle Wildlife & Country Park | North Kessock | Highland | Wildlife |
| Blair Drummond Safari & Leisure Park | Blair Drummond | Stirling | Wildlife Park |
| Bright Water Visitor Centre | Kyleakin, Isle of Skye | Highland | Wildlife |
| Cairngorm Reindeer Centre | Glenmore, Aviemore | Highland | Wildlife Park |
| Camperdown Wildlife Centre | Dundee | Angus | Wildlife Park |
| Deep Sea World | North Queensferry | Fife | Aquarium / Oceanarium |
| Eastwood Butterfly Kingdom | Giffnock | Renfrewshire | Butterfly Farm |
| Edinburgh Butterfly & Insect World | Lasswade | Midlothian | Butterfly Farm |
| Edinburgh Zoo | Edinburgh | Edinburgh | Zoo |
| Glasgow Zoopark | Uddingston | Glasgow | Zoo |
| Glen Finart Deer Farm | Ardentinny | Argyll and Bute | Animal Park |
| Highland Wildlife Park | Kingussie | Highland | Wildlife Park |
| Isle of Mull Angora Rabbit Farm | Bunessan, Isle of Mull | Argyll and Bute | Animal Park |
| Isle of Skye Falconry | Portree, Isle of Skye | Highland | Wildlife |
| Jedforest Deer & Farm Park | Jedburgh | Scottish Borders | Wildlife Park |
| Kathellan Farm Park | Kelty | Fife | Farm Park |
| Kelton Mains Open Farm | Castle Douglas | Dumfries and Galloway | Farm |
| Mabie Farm Park | Mabie, Dumfries | Dumfries and Galloway | Farm Park |
| Oban Rare Breeds Farm Park | Oban | Argyll and Bute | Farm Park |
| Ostrich Kingdom | Birniefield | Fife | Animal Park |
| Praytis Farm Park | Leven | Fife | Farm Park |
| Rankeilour Park & Scottish Deer Centre | Cupar | Fife | Animal Park |
| Scottish Deer Centre | Cupar | Fife | Animal Park |
| Scottish Sea Life Centre, The | Barcaldine, Oban | Argyll and Bute | Aquarium / Oceanarium |
| Sea Life Centre | St Andrews | Fife | Aquarium / Oceanarium |
| Skye Serpentarium Reptile Word | Broadford, Isle of Skye | Highland | Wildlife |
| St Andrews Aquarium | St Andrews | Fife | Aquarium / Oceanarium |
| Tobermory Children's Farm | Tobermory | Argyll and Bute | Farm Park |
| Wildlife Park | Kircudbright | Dumfries and Galloway | Wildlife Park |
